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Re: Anyone w/ experience w/ frozen hooves on valuable calves

Posted: Wed Mar 25, 2015 9:13 pm
by milkmaid
Good luck, thanks for the continued updates.

Re: Anyone w/ experience w/ frozen hooves on valuable calves

Posted: Thu Mar 26, 2015 2:00 pm
by branguscowgirl
Fingers crossed for you!

Re: Anyone w/ experience w/ frozen hooves on valuable calves

Posted: Fri Apr 03, 2015 2:26 pm
by WalnutCrest
Still not too optimistic ...

... but ...

we do have 12 oocytes cooking now and should have results next week.

Re: Anyone w/ experience w/ frozen hooves on valuable calves

Posted: Thu Apr 09, 2015 1:06 pm
by WalnutCrest
Zero.

:(

We'll try again at least once more ...

Re: Anyone w/ experience w/ frozen hooves on valuable calves

Posted: Fri May 01, 2015 10:53 am
by WalnutCrest
Victory!

Four embryos in the tank! We're going again in two weeks.

Re: Anyone w/ experience w/ frozen hooves on valuable calves

Posted: Fri May 01, 2015 11:57 am
by branguscowgirl
Congratulations! :clap:

Re: Anyone w/ experience w/ frozen hooves on valuable calves

Posted: Fri May 01, 2015 7:17 pm
by R V
Congratulations - just curious, but how many oocytes this time to get 4 embryos?

Re: Anyone w/ experience w/ frozen hooves on valuable calves

Posted: Fri May 01, 2015 10:09 pm
by Kathie in Thorp
That all sounds good!

Re: Anyone w/ experience w/ frozen hooves on valuable calves

Posted: Sat May 02, 2015 8:48 am
by WalnutCrest
R V wrote:Congratulations - just curious, but how many oocytes this time to get 4 embryos?


15 ... so two more than last time ...

Re: Anyone w/ experience w/ frozen hooves on valuable calves

Posted: Wed May 06, 2015 4:02 pm
by Ozhorse
Congratulations! Glad you have got some of what you had planned.

How is the girl going herself? Is she still coping with the prosthesis ?

Re: Anyone w/ experience w/ frozen hooves on valuable calves

Posted: Wed Jul 15, 2015 4:13 pm
by WalnutCrest
Long delayed update ...

Yes, Ozhorse, she's handling the prosthetic quite well. Once she started estrus, we really dialed back on the nutrition so that she'd slow her growth rate ... too big of a female would result in more stress on her rear leg which might put an abrupt halt to our IVF aspirations. She's held her condition very well throughout all of this. The fine folks at Trans Ova (Sioux Center, IA) have been absolutely wonderful to work with -- I could not recommend them more highly.

Well, we ended up aspirating Begonia six times. We had a total of 70 oocytes, but only 12 of those converted to embryos. This little lady will be at the butchers within the next 15 days... :( ...the end of a long journey! Two of those aspirations were to reverse-sorted semen (for heifers) ... and we only converted one embryo each of those two times.

...but...the first of those reverse-sorted heifer embryos is getting implanted TODAY! Here's to hoping it sticks!

Thanks to everyone for their encouragement, questions and well-wishes. :)

...now, it's time to break out the checkbook and add up the total damage!

Re: Anyone w/ experience w/ frozen hooves on valuable calves

Posted: Wed Jul 15, 2015 5:10 pm
by Ozhorse
Wow, you have been very dedicated. A very high tech way of producing cattle!

Do keep us posted on how the embryo transplant goes.

I am sure the whole exercise is very interesting, cheque book wise.

Re: Anyone w/ experience w/ frozen hooves on valuable calves

Posted: Sun Jul 26, 2015 6:05 pm
by WalnutCrest
The embryo tech decided the recip wasn't 'perfect' so we put another embryo in instead of the one from Begonia...

Quite separately, we asked Trans Ova to send us some current pictures of Begonia -- she's turned into quite a little star up there -- and one of the interns was all to happy to oblige. One from each side and a 'glamour shot'.

Enjoy -- and thanks again for all the great questions and encouragement along the way.

Image

Image

Image

Re: Anyone w/ experience w/ frozen hooves on valuable calves

Posted: Sun Jul 26, 2015 7:07 pm
by Ozhorse
She is a nice looking animal, thanks for the photos.

Re: Anyone w/ experience w/ frozen hooves on valuable calves

Posted: Tue Aug 04, 2015 11:05 pm
by WalnutCrest
Great news -- Begonia will be getting AI'd next week!

One of the head vets who was responsible for her daily care at Trans Ova approached us about partnering on her. The vet and her husband (also a vet) will attend to her and will take care of the wound and her well-being in an effort to get a calf or five on the ground (plus maybe some more aspirating down the road) over the next few years. We have no expectations other than she's good enough to try. And, to make it a complete full circle, the vet (Dr. Jolly in AR) who was overseeing the development of her low-cost prosthetics has agreed to try to put together another one as she's weeks away from being too big for the one she's been on since last fall.

Good stuff. Very good stuff!